Fig. 4

RNF7 overexpression reduces apoptosis and enhances glycolysis through the JAK/STAT3 signaling pathway. A–F ACHN cells transduced with RNF7-overexpressing lentivirus or blank lentivirus (vector) were treated with 10 µM AG490. A Cell viability was measured by CCK-8 assay. Flow cytometry was used to analyze B cell apoptosis and D ECAR level. C Lactate release and E ATP content were determined by biochemical analyses. F The protein levels of HK2, GLUT1, cleaved PARP1, cleaved caspase-3, STAT3, and p-STAT3 were determined by Western blot. Experiments performed in triplicate; data expressed as mean ± SD (n = 3). **P < 0.01, ***P < 0.001 compared with vector. ^###P < 0.001 compared with RNF7