Fig. 6

SOCS1 neutralizes the RNF7-mediated effects on apoptosis and glycolysis in ccRCC cell lines. ACHN cells were transduced with the indicated plasmids, and flow cytometry was performed to assess (A, B) cell apoptosis and C ECAR level. Biochemical assays were performed to measure D lactate release and E ATP content. F The protein levels of RNF7, SOCS1, HK-2, GLUT1, STAT3, and p-STAT3 were determined by Western blot. Experiments performed in triplicate; data expressed as mean ± SD (n = 3). ***P < 0.001 compared with vector. ^###P < 0.001 compared with RNF7