Fig. 7

RNF7 regulates the sensitivity of ccRCC cells to sunitinib. A, C Caki-1 cells transduced with RNF7 shRNA or control scramble shRNA (shNC) and B, D ACHN cells transduced with RNF7-overexpressing lentivirus or blank lentivirus (vector) were treated with sunitinib (1 μM) or vehicle in absence or presence of 10 µM AG490 for 24 h, and cell apoptosis was subsequently measured (n = 3 per group). ACHN cells were stably transduced with RNF7-overexpressing lentivirus or blank lentivirus (vector), then these cells were subcutaneously injected into nude mice (n = 5 per group). After 12 days, the mice received an intraperitoneal injection of sunitinib (20 mg/kg) every other day. On day 33, the E tumor volume and F weight were measured. G, H Xenograft tumors with TUNEL staining. Scale bar: 50 µm. I The protein levels of RNF7, STAT3, and p-STAT3 in xenograft mouse tumors were determined by Western blot. Experiments performed in triplicate; data expressed as mean ± SD (n = 3 or 5). **P < 0.01, ***P < 0.001 compared with vehicle or vehicle + vector. ^###P < 0.001 compared with sunitinib + shNC or sunitinib + vector. ^ΔΔΔP < 0.001 compared with sunitinib + shRNF7 or sunitinib + RNF7