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Fig. 8 | Cellular & Molecular Biology Letters

Fig. 8

From: RNF7 inhibits apoptosis and sunitinib sensitivity and promotes glycolysis in renal cell carcinoma via the SOCS1/JAK/STAT3 feedback loop

Fig. 8

STAT3 activation transcriptionally regulates the expression of RNF7. A A luciferase reporter assay was performed to evaluate the activity of the RNF7 promoter in Caki-1 cells treated with AG490 or vehicles. B Quantitative RT-PCR analysis of the effects of AG490 (10 μM) on RNF7 mRNA expression. C The STAT3 binding site in the RNF7 promoter was predicted by the JASPAR algorithm. D The ChIP assay results indicate that STAT3 bound to the RNF7 promoter. E Schematic diagram of the primers used in the ChIP analysis. 3′-UTR served as a negative control (NC). BS, binding site; BSm, mutant binding site. Experiments performed in triplicate; data expressed as mean ± SD (n = 3). ***P < 0.001 compared with vehicle or IgG

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