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Fig. 1 | Cellular & Molecular Biology Letters

Fig. 1

From: HCMV-miR-US33-5p promotes apoptosis of aortic vascular smooth muscle cells by targeting EPAS1/SLC3A2 pathway

Fig. 1

HCMV promotes apoptosis by upregulating HCMV-miR-US33-5p. A qRT-PCR analysis of HCMV-miR-US33-5p expression level in HA-VSMCs infected with different viruses. B CCK-8 assay showed that HCMV AD169 virus infection inhibited HA-VSMC proliferation, while ultraviolet inactivation of the virus (UV) or the AD169 (ΔUS33) virus showed a greatly reduced inhibitory effect on HA-VSMC proliferation. C–E Flow cytometry assay (C), caspase-3 activity assay (D), and Western blot analysis of cleaved caspase-3 (E) indicated that HCMV AD169 virus infection promoted HA-VSMC apoptosis, while UV-inactivated virus (UV) or AD169 (ΔUS33) virus showed a greatly reduced stimulatory effect on HA-VSMC apoptosis. Western blot was performed three times; representative images are presented. Numbers above lanes indicate the relative protein level, normalized relative to control samples. *P < 0.05, **P < 0.01, ***P < 0.001 (n = 3, ANOVA analysis)

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Cellular & Molecular Biology Letters

ISSN: 1689-1392

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