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Fig. 4 | Cellular & Molecular Biology Letters

Fig. 4

From: HCMV-miR-US33-5p promotes apoptosis of aortic vascular smooth muscle cells by targeting EPAS1/SLC3A2 pathway

Fig. 4

Downregulating SLC3A2 promotes HA-VSMC cell apoptosis. HCMV-miR-US33-5p was transfected into HA-VSMCs. A RNA sequencing data showing differential expression of SLC3A2 between HCMV-miR-US33-5p and control groups. B, C SLC3A2 expression in HA-VSMCs. D, E SLC3A2 expression in HA-VSMCs after silencing SLC3A2. F CCK-8 assay showed that transfection of siRNA targeting SLC3A2 inhibited HA-VSMC proliferation. G–I Flow cytometry assay (G), caspase-3 activity assay (H), and Western blot analysis of cleaved caspase-3 (I) showed that transfection of siRNA targeting SLC3A2 promoted HA-VSMC apoptosis. J–M Western blot analysis of the effect of SLC3A2 siRNA on p-FAK (J), p-Akt (K), pY20CAS (L), and GTP-Rac (M) in HA-VSMCs. Western blot was performed three times; representative images are presented. Numbers above lanes indicate the relative protein level, normalized relative to control samples. *P < 0.05, ***P < 0.001, ****P < 0.0001 (n = 3, ANOVA analysis)

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Cellular & Molecular Biology Letters

ISSN: 1689-1392

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