Fig. 5

lncRNA SNHG12 bound to HuR to increase mRNA stability and expression of USP8. A Probability of HuR binding to USP8 was predicted via the RPISeq database; B the binding relationship between USP8 and HuR was analyzed using RIP assay; C–F USP8 expression in NSCLC tissues and cells was measured via RT-qPCR and western blotting; G correlation between lncRNA SNHG12 and USP8 was analyzed via Pearson correlation analysis; H, I USP8 expression in NSCLC cells was measured via RT-qPCR and western blotting; J half-life period of USP8 mRNA in NSCLC cells was assessed via RT-qPCR. N = 65, cell experiments were performed three times, *P < 0.05, **P < 0.01. Data in A, B, D–F, and H–J are represented as mean ± standard deviation. Pairwise comparisons in C and D were analyzed using t-test; multigroup comparisons in B, E, and F were analyzed using one-way ANOVA, and in E, F, and H–J were analyzed using two-way ANOVA, followed by Tukey’s multiple comparison test