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Fig. 7 | Cellular & Molecular Biology Letters

Fig. 7

From: Molecular mechanism of lncRNA SNHG12 in immune escape of non-small cell lung cancer through the HuR/PD-L1/USP8 axis

Fig. 7

USP8 overexpression attenuated the inhibition of silencing lncRNA SNHG12 on the immune escape of NSCLC. A549 or H1299 cells were transfected with USP8 pcDNA3.1 (pc-USP8), with NC pcDNA3.1 (pc-NC) as the negative control. A mRNA level of USP8 in NSCLC cells was measured via RT-qPCR; B protein expression of USP8 and PD-L1 was detected via western blotting; C mRNA level of PD-L1 was measured via RT-qPCR; D ubiquitination level of PD-L1 in NSCLCs was determined via western blotting; A549 or H1299 cells in different treatment groups were co-cultured with isolated PBMCs. E PBMC proliferation was observed via Ki67 staining; F percentage of CD8+ T cells in PBMCs was determined via flow cytometry; G concentrations of TNF-α, IFN-γ, IL-10, and TGF-β were determined via ELISA; H cytotoxicity of PBMCs was detected via LDH cytotoxicity assay. Cell experiments were performed three times, *P < 0.05, **P < 0.01. Data are represented as mean ± standard deviation. Multigroup comparisons in A–C and E–H were analyzed using two-way ANOVA, followed by Tukey’s multiple comparison test

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