Fig. 4From: Effect of chronic intermittent hypoxia-induced HIF-1α/ATAD2 expression on lung cancer stemnessEffect of decreased ATAD2 levels on mtROS production in lung cancer cells. A ATAD2 mRNA expression in lung cancer cells transfected with the ATAD2 overexpression plasmid. B Cellular mtROS accumulation in A549 and SPC-A1 cells assessed using flow cytometry analysis. C, D Nuclei are stained with Hoechst (blue), and mitochondria ROS are stained using MitoSOX-red in A549 and SPCA1 cells. Merged panels demonstrate the number of MitoSOX-red positive cells among the total cells. Scale bar = 100 μm. Error bars indicate mean ± SD. All experiments performed in triplicate. *p < 0.05Back to article page