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Fig. 4 | Cellular & Molecular Biology Letters

Fig. 4

From: Integrated analysis of tRNA-derived small RNAs in proliferative human aortic smooth muscle cells

Fig. 4

AS-tDR-000067 suppression inhibits HASMC proliferation by elevating p53 levels. A EdU incorporation assay of HASMCs transfected with ASO-Control or ASO-AS-tDR-000067. Blue fluorescence (Hoechst 33342) indicates cell nuclei, while red fluorescence (EdU) represents HASMCs with DNA synthesis. Scale bar 10 µm; B Relative EdU-positive HASMCs. Data expressed as the ratio of EdU-positive HASMCs to total ones. Data shown as mean ± SD of at least three independent experiments, ****p < 0.0001 versus ASO-Control group; C Western blot analysis of p53 in HASMCs transfected with ASO-Control or ASO-AS-tDR-000067, with GAPDH as control. Data shown as mean ± SD of three independent experiments, *p < 0.05 versus ASO-Control group; D Schematic of binding sites of AS-tDR-000067 to p53 promoter; E Verification of p53 promoter as a target of AS-tDR-000067 utilizing the dual-luciferase reporter gene assay. Data shown as mean ± SD of at least three independent experiments, *p < 0.05

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