Fig. 1
From: Raman microspectroscopy fingerprinting of organoid differentiation state
FGF2 promotes proacinar and inhibits ductal differentiation in embryonic salivary gland organoids. A Illustration of salivary gland organoid creation from murine embryonic salivary gland cells, which are separated for regulated recombination in Matrigel. Keratin-7 (K7)-positive ductal cells are shown in red, aquaporin-5 (AQP5)-positive pro-acinar cells are shown in green, and mesenchymal stromal cells are shown in yellow. Created with Biorender.com. B Representative images show that, while all organoids express the pan-epithelial marker EpCAM (white) and some level of the ductal marker K7 (red), the elaboration of AQP5+ cells (green) is restricted to branched organoids treated with FGF2. Nuclei are stained with DAPI. (C–E) Quantification of microscopy images with relative area ratios for AQP5 to DAPI, K7 to DAPI, and AQP5 to K7. Error bar represents 95% confidence interval, n = 3 technical replicates; asterisk indicates significance with p < 0.05 (one-way ANOVA with Tukey’s post hoc test) (statistical summary in Additional file 1: Table S1–S3)