Fig. 2

Development of Raman confocal microspectroscopy method to evaluate salivary gland organoids. A Quantification of organoid size based on area. n = 7 technical replicates across three experiments (statistical summary in Additional file 1: Table S4). B Representative images of control and growth factor-treated organoids prior to Raman imaging, with the cell-dense, largely epithelial regions highlighted in zoomed panel. Scale bar, 500 µm (panel), 250 µm (zoomed region). C Visualization of the Raman confocal microspectroscopy method for identification of cell-dense regions. This method allows the separation of cell dense-organoid regions from Matrigel-dominant, or stromal, regions, which can be done either manually by peak comparison or with a multivariate SVD approach