Fig. 6

Unknown organoids identified using SVD analysis of Raman spectra. Raman spectra derived from the unknown (blinded) datasets of cell-dense organoid regions were subjected to SVD analysis with known FGF2 (green), EGF (blue), and control (gray) datasets (derived from Fig. 3). One representative plot is shown for each unknown, which demonstrates codistribution with a known treatment. A SVD plot of unknown 1 with known EGF and FGF2 spectra. B Percentage of population of unknown 1 codistributed with FGF2 or EGF. C SVD plot of unknown 2 with known EGF and control spectra. D Percentage of population of unknown 2 codistributed with EGF or control. E SVD plot of unknown 3 with known EGF and control spectra. F Percentage of population of unknown 2 codistributed with EGF or control (specific assignments of unknown samples detailed in Table 2). Additional SVD comparisons for unknowns 1–3 are provided in Additional file 1: Fig. S2–S4, respectively