Fig. 3

Maturation of miR-221/222 was promoted by METTL3 in an m6A-dependent manner. A qRT-PCR detected the expression of miR-221 and miR-222 in Ang-II-induced NRCMs. n = 3. B qRT-PCR detected the expression of pri-miR-221 and pri-miR-222 in Ang-II-induced NRCMs. n = 3. C METTL3 was overexpressed and inhibited respectively after transfecting plvx-METTL3 or shMETTL3 into NRCMs. qRT-PCR detected the expression of miR-221 and miR-222. n = 3. D Detection of pri-miR-221 binding to DGCR8 or m6A modification by immunoprecipitation experiments in control and METTL3 overexpression NRCMs. n = 3. E Detection of pri-miR-222 binding to DGCR8 or m6A modification by immunoprecipitation experiments in control and METTL3 overexpression NRCMs. n = 3. *P < 0.05, **P < 0.01, ***P < 0.001