Fig. 6

DKK2 was the direct target of miR-221/222 in regulating Wnt signaling in NRCMs. A The miR-221/222 binding site in the 3′-UTR of DKK2 predicted by TargetScan database. B qRT-PCR detected the expression of DKK2 in NRCMs stimulated with Ang-II. n = 3. C Western blot detected the expression of DKK2 in NRCMs stimulated with Ang-II. n = 3. D qRT-PCR detected the expression of DKK2 in NRCMs transduced with miR-221 mimics and inhibitors. n = 3. E Western blot detected the expression of DKK2 in NRCMs transduced with miR-221 mimics and inhibitors. n = 3. F qRT-PCR detected the expression of DKK2 in NRCMs transduced with miR-222 mimics and inhibitors. n = 3. G Western blot detected the expression of DKK2 in NRCMs transduced with miR-222 mimics and inhibitors. n = 3. H Predictive miR-221/222 binding sites in the 3′-UTR of DKK2 mRNA. Dual-luciferase reporter assays demonstrated that DKK2 was a direct target of miR-221/222. I Western blot detected the expression of β‐catenin and c-Myc in Ang-II-induced NRCMs with miR-221/222 inhibition and DKK2 knockdown. n = 3. J qRT-PCR detected the expression of c-Myc in Ang-II-induced NRCMs with miR-221/222 inhibition and DKK2 knockdown. n = 3. *P < 0.05, **P < 0.01, ***P < 0.001