Fig. 7

AAV9-delivery system carrying shMETTL3 alleviates the degree of hypertrophy in mice. A Workflow of animal research. B Western blot detected the expression of METTL3 in AAV9-shMETTL3 transfected with mice infused with Ang-II. n = 3. C qRT-PCR detected the expression of biomarkers, including ANP and BNP, in the heart of the three groups. n = 4. D Cardiac color Doppler ultrasound evaluated the indexes of cardiac structure, including LVAWs, LVAWd, EF, and FS. n = 7 in shNC group, n = 8 in Ang-II + shNC group, n = 7 in Ang-II + shMETTL3 group. E Representative heart gross morphology from the mice among three groups. n = 5. F Histological sections stained with H&E and WGA from mice of the three groups. n = 5. Scale bars, 50 μm. *P < 0.05, **P < 0.01, ***P < 0.001