Fig. 5
From: WTAP-mediated N6-methyladenosine modification of NLRP3 mRNA in kidney injury of diabetic nephropathy
WTAP overexpression promotes cell pyroptosis and pro-inflammatory factor release in HK-2 cells by targeting NLRP3. A, B Cell pyroptosis; C lactate dehydrogenase activity; D, E expression of NLRP3, pro-caspase-1, active caspase-1, GSDMD, and GSDMD-N; (F, G) release of IL-18, IL-1β, TNF-α, and IL-6 in HK-2 cells with or without WTAP overexpression and NLRP3 knockdown (n = 3). ***P < 0.001 compared with vector + siNC. One-way analysis of variance was used to analyze the data among multiple groups, followed by Tukey’s post hoc test. ##P < 0.01, ###P < 0.001 compared with WTAP-OE + siNC