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Fig. 2 | Cellular & Molecular Biology Letters

Fig. 2

From: Alpha-smooth muscle actin-positive cancer-associated fibroblasts secreting osteopontin promote growth of luminal breast cancer

Fig. 2

Characterization of CAFs isolated from patients with BC. A Representative pictures of immunofluorescence staining for α-SMA (red) in α-SMAlow and α-SMAhigh CAFs; DAPI (blue) was used for nuclei counterstaining. B Representative pictures of MCF7 cells growing in 3D-Matrigel cultures, when treated with conditioned medium from α-SMAlow or α-SMAhigh CAFs, and BJ or DMEM as a control. C Quantification of colony area fold change. Colony area was determined with ImageJ software. The values presented are mean ± SD from three independent experiments (n = 2 technical replicates). Unpaired Student’s t-test test was applied, ***p < 0.0001 calculated versus control (DMEM)

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