Fig. 4

ROR2 regulates the expression of MDM2, p53, and Bcl2-family proteins through the hyperactivation of ERK. A PLX inhibited MDM2 levels and increased p53 in A375-ROR2 cells. The cells were treated with 10 µM PLX for the indicated time. The graphs show the mean ± SD of each protein’s levels normalized to the corresponding loading control and expressed as the fold change (FC) relative to untreated cells. B Bcl2 proteins are regulated by the MAPK/ERK pathway in A375-ROR2 cells. The cells were treated with 10 µM PLX for the indicated times. The graphs show the mean ± SD of Bcl-xL, Mcl-1, and Bax normalized to the corresponding loading control and expressed as the fold change (FC) relative to untreated cells. Statistical signifcance was tested by a one-tailed Student’s t-test or ANOVA as appropriate (n = 3). ***p < 0.0001, n.s. not significant