Fig. 3
Molecular mechanism of DLX4 isoforms BP1 and DLX7 in leukemogenesis. a The flow chart of the molecular mechanism experiment. b Volcano plot of DEGs between K562-BP1 and K562-NC cells. c KEGG analysis of differentially expressed genes between K562-BP1 and K562-NC cells. d The top three motifs analyzed with K562-BP1 cells by ChIP-Seq. e, f Potential downstream targets by Venn analysis of ChIP-Seq and RNA-Seq. g Validation of the selected gene expression by RT-qPCR. h The proliferation ability of K562-BP1 cells affected by BP1 downstream target gene knockdown. Both K562-BP1-siRREB1 and K562-BP1-siSGMS1-AS1 cells showed significantly reduced proliferation rate compared with K562-BP1-siNC cells. i Flow chart of the molecular mechanism experiment. j Volcano plot of DEGs between K562-DLX7 and K562-NC cells. k KEGG analysis of DEGs between K562-DLX7 and K562-NC cells. l The top one motif analyzed with K562-DLX7 cells by ATAC-Seq. m, n Potential downstream targets by Venn analysis of ATAC-Seq and RNA-Seq. o Validation of selected gene expression by RT-qPCR. p Proliferation ability of K562-DLX7 cells affected by DLX7 downstream target gene knockdown. Both K562-DLX7-siPTPRB and K562-DLX7-siNEAT1 cells showed markedly increased proliferation rate compared with K562-DLX7-siNC cells. *P < 0.05; **P < 0.01; ***P < 0.001