Skip to main content
Fig. 4 | Cellular & Molecular Biology Letters

Fig. 4

From: Granulin as an important immune molecule involved in lamprey tissue repair and regeneration by promoting cell proliferation and migration

Fig. 4

Lr-PGRN-S1 promotes cell proliferation and migration. A, B rL-PGRN-S1 promoted HEK293T cell and HUVEC proliferation. HEK293T cells and HUVECs were treated with rLr-PGRN-S1 of different concentrations. The viability of the cells was detected using the CCK-8 assay. C Wounds were produced by scratching the bottom of the cell culture plate using a pipette tip. Cells were incubated with IPC for up to 48 h. To observe the wound healing effect, the area around the wounds was assessed at indicated time points. PBS was used as control. D Line chart showing the statistics of the results in C. E Transwell assay showing promotion of migration in rL-PGRN-S1-treated HUVECs; F graph of rL-PGRN-S1 promotion of HUVEC migration. G Transwell assay showing promotion of migration in rL-PGRN-S1-treated lamprey supraneural body. H Statistical graph of rL-PGRN-S1. I Effect of rL-PGRN-S1 on expression of chemokine LECT2 as determined by qRT-PCR. J Effect of rL-PGRN-S1 on expression of chemokine LECT2 as determined by western blot. The lower histogram shows the statistics of western blot. The probability of statistical differences between experimental groups was determined by Student’s t-test. All data presented as mean ± SD based on three independent samples with three replicates per sample. *P < 0.05, **P < 0.001, ***P < 0.0001

Back to article page