Fig. 5

Expression of Lr-pgrn-s1 after lamprey skin and spinal cord injury. A Expression of Lr-pgrn-s1 in lamprey skin lesions 1 and 2 days after injury. B Expression of Lr-pgrn-s1 in lamprey 6 h, 1 day, and 3 days after spinal cord injury by qRT-PCR. C Tissue-wise localization of Lr-PGRN-S1 1d after skin injury by immunohistochemistry. Pictures taken at ×40 magnification. D Immunohistochemistry analysis shows Lr-pgrn-s1 was knocked down 6 h after lamprey spinal cord injury. Results under ×20 and ×40 microscopy in sham, NC (normal control), and Lr-PGRN-S1 groups. E qRT-PCR analysis of genes in MAPK, Notch, Wnt, and TGF-β signaling pathways. Statistical differences between experimental groups were detected by Student’s t test. All data presented as mean ± SD based on three independent samples with three replicates per sample. ns nonsignificant, *P < 0.05, **P < 0.001, ***P < 0.0001