Fig. 4

RNA-seq revealed cotreatment with d-borneol and CDDP enriched in cell cycle. A Volcano plot displaying the dysregulated genes between CDDP + Bor and CDDP groups; the red points represent significantly upregulated genes, and the green points represent significantly downregulated genes in CDDP + Bor; B heatmap showing differentially expressed genes in CDDP + Bor treatment groups compared with CDDP groups; C GO enrichment analysis of biological processes of downregulated genes in CDDP + Bor treated group; D KEGG pathway enrichment analysis of downregulated differentially expressed genes in CDDP + Bor group. Each group has three biological replicates; E–H, M mRNA expression level of cyclin A2 (E) and cyclin D3 (F) and protein level of cyclin A2 (G, M) and cyclin D3 (H, M); I, J mRNA expression level of CDK2 (I) and CDK6 (J); K–M western blotting and quantification analysis of CDK2 and CDK6. All data are presented as mean ± SD (n = 3, *P < 0.05, compared with Con; ^#P < 0.05, compared with CDDP group)