Table 1 Primer sequences for real-time PCR assays
Name | Forward primer sequence (5′–3′) | Reverse primer sequence (5′–3′) |
|---|---|---|
GAPDH | CAGGAGGCATTGCTGATGAT | GAAGGCTGGGGCTCATTT |
ABCB1 | GATTGCTCACCGCCTGTCCAC | CGTGCCATGCTCCTTGACTCTG |
CCNA2 | GCCAGACATCACTAACAGTATGAGAGC | GCACTGACATGGAAGACAGGAACC |
cyclinD3 | TGCCACAGATGTGAAGTTCATT | CAGTCCGGGTCACACTTGAT |
CDK2 | ATGAAGATGGACGGAGCTTGTTATCG | CTGGCTTGGTCACATCCTGGAAG |
CDK6 | CGAACAGACAGAGAAACCAAAC | CTCGGTGTGAATGAAGAAAGTC |
p21 | CATGTGGACCTGTCACTGTCTTGTA | GAAGATCAGCCGGCGTTG |
p27 | CTAACTCTGAGGACACGCATTT | TTGAGTAGAAGAATCGTCGGTT |
Bax | GATGCGTCCACCAAGAAGCTGAG | CACGGCGGCAATCATCCTCTG |
Bcl-2 | TCGCCCTGTGGATGACTGAGTAC | TCAGAGACAGCCAGGAGAAATCAAAC |
Caspase-3 | GTGGAGGCCGACTTCTTGTATGC | TGGCACAAAGCGACTGGATGAAC |