Fig. 2

circ_0025033 knockdown hampered the malignant behavior of ovarian cancer cells in glutamine metabolism. A–M SKOV3 and A2780 cells were transfected with si-NC or si-circ_0025033. A qRT-PCR analysis of circ_0025033. B and C CCK-8 and EdU analysis of cell viability and DNA synthesis. D Apoptosis rate analyzed by flow cytometry analysis. E and F Cell invasion measured by transwell assay. G Tube formation assay for evaluating angiogenesis activity. H–J Western blot analysis of c-Myc and MMP9 levels. K–M Glutamine consumption, α-ketoglutarate production, and glutamate production measured via glutamine, α-ketoglutarate, and glutamate assay kits, respectively. **P < 0.01, ***P < 0.001, ****P < 0.0001