Fig. 2
From: SESN2 prevents the slow-to-fast myofiber shift in denervated atrophy via AMPK/PGC-1α pathway
SESN2 protected against muscle atrophy and myofiber type transition after denervation. A Western blot analysis of SESN2 expression in GAS post-denervation. B Western blot analysis confirming the expression of SESN2 and MHC. C Appearance and wet weight ratio of GAS in different groups. D Quantification of muscle fiber diameter by immunofluorescence staining of WGA. Scale bar, 50 μm. E Western blot analysis of proteins related to muscle fiber type. F Immunofluorescence analysis of fiber type composition in denervated GAS. Scale bar, 50 μm. G, H Relative ATP content and metabolic enzyme activities (LDH and SDH) in GAS. Data presented as mean ± SD. n = 6. *P < 0.05 versus control (week 0 or sham operation group). #P < 0.05 versus denervation group. Den denervation, Con control