Fig. 3
From: SESN2 prevents the slow-to-fast myofiber shift in denervated atrophy via AMPK/PGC-1α pathway
Rotenone induced slow-to-fast fiber type shift in C2C12 myotubes. A Mitochondrial ROS levels in C2C12 cells were detected by MitoSOX Red labeling. Scale bar, 10 μm. B Immunofluorescence analysis of slow and fast MHC. Scale bar, 50 μm. C Western blot analysis of slow and fast MHC. D, E Detection of relative ATP content and metabolic enzyme activity (LDH and SDH) in C2C12 myotubes. Data presented as mean ± SD. *P < 0.05 versus control (0 nM rotenone treatment). Rot rotenone