Fig. 5
From: SESN2 prevents the slow-to-fast myofiber shift in denervated atrophy via AMPK/PGC-1α pathway
SESN2 prevented slow-to-fast shift in rotenone-treated C2C12 myotubes via AMPK/PGC-1α pathway. A Western blot analysis of the regulatory effect of SESN2 on the activity of AMPK/PGC-1α signaling. B Western blot analysis of C2C12 myotubes treated with rotenone and AICAR (an AMPK activator) to confirm the role of AMPK/PGC-1α signaling in myofiber type transition. C Immunofluorescence analysis of slow and fast MHC. Scale bar, 50 μm. D, E Detection of relative ATP content and metabolic enzyme activity (LDH and SDH) in C2C12 myotubes. Data presented as mean ± SD. *P < 0.05 versus control or siNC group. #P < 0.05 versus rotenone treatment group. Rot rotenone, Con control