Fig. 7
From: SESN2 prevents the slow-to-fast myofiber shift in denervated atrophy via AMPK/PGC-1α pathway
AMPK/PGC-1α signaling prevented slow-to-fast myofiber shift and protected against muscle atrophy upon denervation. A Western blot analysis confirmed the role of AMPK/PGC-1α signaling in myofiber type transition in denervated GAS. B Immunofluorescence analysis of fiber type composition in denervated GAS. Scale bar, 50 μm. C, D Relative ATP content and metabolic enzyme activities (LDH and SDH) in GAS with different treatment. E Appearance and wet weight ratio of GAS in different groups. F Western blot analysis confirmed the expressions of SESN2 and MHC. G Quantification of muscle fibers diameter by immunofluorescence staining of WGA. Scale bar, 50 μm. Data presented as mean ± SD. n = 6. *P < 0.05 versus denervation + shNC group. #P < 0.05 versus denervation + shSESN2 group. Den denervation