Fig. 2

Hsa-miR-27b inhibits the proliferation and migration of HCC cells in vitro. A Endogenous expression of hsa-miR-27b in WRL68 and HCC cell lines. Data were normalized to U6 and are shown as fold change compared with HLF cells (n = 3). B Overexpression or knockdown of hsa-miR-27b in HCC cells using hsa-miR-27b mimics or inhibitors, respectively, as measured by qRT-PCR. Data were normalized to U6 and are shown as fold change relative to the control cells (n = 3, Mann–Whitney test). C The proliferation of the indicated cells with overexpression or knockdown of hsa-miR-27b as measured by CCK-8 assays (n = 5, Kruskal–Wallis test). D Representative images of the EdU incorporation assay (left). Scale bar: 100 μm; quantification of EdU-positive cells (right) (n = 3, Mann–Whitney test). E Representative images (top) and quantification (bottom) of cells that migrated or invaded in the indicated groups (n = 3, Mann–Whitney test). Scale bar, 200 μm. F Representative pictures of phalloidin staining for F-actin (red) in the indicated cells. DAPI was used to show the nucleus (blue). Scale bar, 20 μm. G Western blot analysis for EMT markers as indicated. GAPDH was used as a loading control. All experiments were repeated three times. *p < 0.05. **p < 0.01. Data are shown as mean ± SD. NC, negative control