Fig. 3

Hsa-miR-27b inhibits the growth and lung metastasis of HCC cells in vivo. A qRT-PCR analysis of hsa-miR-27b stable overexpression or knockdown efficacy in the indicated cells. Data were normalized to U6 and are shown as fold change relative to the control cells (n = 3, Mann–Whitney test). B Macroscopic images of subcutaneous tumours from mice (n = 7) bearing hsa-miR-27b-overexpressing MHCC97H cells or hsa-miR-27b-knockdown Hep3B cells (left); quantification of the volumes and weights of subcutaneous tumours in the indicated groups (right); unpaired t-test. C Representative haematoxylin and eosin (H&E) staining and IHC of Ki67 in subcutaneous xenografts. Quantification of staining scores was calculated (n = 7, unpaired t-test); Scale bar, 200 μm. D Lung metastasis experiments were performed in nude mice with the indicated cells. D1 and D2 Representative macroscopic and H&E staining images of lung metastases. D3 Quantification of visible tumour nodules. MHCC97H: control group (n = 7); hsa-miR-27b group (n = 7); unpaired t-test. Hep3B: control group (n = 7); KD hsa-miR-27b group (n = 7); Mann–Whitney test. D4 Lung metastatic incidence in different groups; *p < 0.05. **p < 0.01. Data are shown as mean ± SD. ctrl, control; KD, knockdown