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Fig. 7 | Cellular & Molecular Biology Letters

Fig. 7

From: circ_0086296 induced atherosclerotic lesions via the IFIT1/STAT1 feedback loop by sponging miR-576-3p

Fig. 7

circ_0086296 is transcriptionally regulated by STAT1 in HUVECs. A UHRF2 level in HUVECs infected with sh-STAT1 vector and overexpressed STAT1 vector was detected by qRT-PCR. **p < 0.001 versus the shNC group. ##p < 0.001 versus the OE-NC group. B Schematic diagram exhibiting the binding sequences in the promoter area of circ_0086296 for STAT1. C The luciferase activities were measured using luciferase reporter assay in HUVECs infected with the UHRF2 vector (wild type and mutant) and overexpressed STAT1 vector. D The enrichment of potential binding sites were detected using the STAT1 antibody by ChIP–PCR. E Relative circ_0086296 expression was measured in HUVECs infected with sh-STAT1 vector and overexpressed STAT1 vector. **p < 0.001 versus the shNC group. ##p < 0.001 versus the OE-NC group. F Detection of migration potential of HUVECs infected with overexpressed STAT1 vector and sh-circ_0086296, miR-576-3p mimics, or sh-IFIT1. &&p < 0.001 versus the sham group, **p < 0.001 versus the pcDNA group, ##p < 0.001 versus the relative control group. G Detection of the vasculogenic capacity of HUVECs infected with overexpressed STAT1 vector and sh-circ_0086296, miR-576-3p mimics, or sh-IFIT1. &&p < 0.001 versus the sham group, **p < 0.001 versus the pcDNA group, ##p < 0.001 versus the relative control group

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