Fig. 9

circ_0086296 induced the atherosclerotic lesion phenotype of HUVECs via exosomes. A Exosomes originating from plasma from patients with atherosclerosis were identified via scanning electron microscopy (SEM). B The size range of isolated extracellular vesicles (EVs) was calculated via Nanosight. C EV marker expression was revealed by western blot. D, E circ_0086296 (D) and miR-576-3p (E) expression in EVs originating from plasma from patients with atherosclerosis was detected via qRT-PCR. **p < 0.001 versus healthy group. F Exosomes originating from ox-LDL-treated HUVECs were identified via SEM. G The size range of isolated EVs was calculated via Nanosight. H The levels of EV markers were revealed by western blot. I circ_0086296 expression in EVs originating from ox-LDL-treated HUVECs was measured via qRT-PCR. **p < 0.001 versus sham group. J miR-576-3p expression in EVs originating from ox-LDL-treated HUVECs was detected via qRT-PCR. K Exosomes derived from the fluorescently labeled cells were taken up by HUVECs. L Migration potential of HUVECs treated with circ_0086296-OE and sh-circ_0086296 cell-derived exosomes was detected via Transwell assay. Scale bar, 100 μm. **p < 0.001 versus the shNC EV group, ^#p < 0.05, ^##p < 0.001 versus the OE-NC EV group. M Detection of the vasculogenic ability of HUVECs treated with circ_0086296-OE and sh-circ_0086296 cell-derived exosomes. *p < 0.05, **p < 0.001 versus the shNC EVs group, ^#p < 0.05, ^##p < 0.001 versus OE-NC EVs group