Fig. 3

Pou3f1 knockdown ameliorated LPS-induced inflammation in macrophages. (A-D) The mRNA and protein levels of Pou3f1 in RAW264.7 cells and BMDMs in response to LPS were determined by qPCR and Western blot. (E) The relative mRNA levels of IL-1β, IL-6, TNF-α and MCP-1 were determined by qPCR. (F) The protein levels of iNOS and COX-2 were detected using Western blot. (G-H) Flow cytometry analysis and quantification results for the fluorescence intensity of ROS. n = 3. Values were mean ± SD. *, p < 0.05; **, p < 0.01