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Fig. 6 | Cellular & Molecular Biology Letters

Fig. 6

From: Pou3f1 mediates the effect of Nfatc3 on ulcerative colitis-associated colorectal cancer by regulating inflammation

Fig. 6

Pou3f1 knockdown inhibited inflammation in colons of UC-CRC mice. (A) The concentration of IL-1β and MCP-1 was measured using ELISA. (B) The relative mRNA levels of IL-1β and MCP-1 were determined by qPCR. (C-E) The PGE2 concentration, MPO activity, and ROS production were measured using commercial kits. (F) Western blot analysis for iNOS and COX-2 protein levels. (G) Representative immunohistochemical images of COX-2 in colons. (H-I) Representative immunofluorescent images of F4/80 (red) and quantification results. DAPI (blue) was used to stain the cell nuclei. Data were from n = 6 mice per group. Values were mean ± SD. **, p < 0.01

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