Fig. 2

LARRPM repressed proliferation, induced apoptosis and inhibited migration and invasion of LUAD cells. a LARRPM expression in A549 and H1975 cells with LARRPM stable overexpression and A549 and H1975 control cells was detected by qRT-PCR. b, c Cell proliferation of A549 and H1975 cells with LARRPM stable overexpression and A549 and H1975 control cells was detected using CCK-8 assays (b) and EdU incorporation assays (c). Scale bar: 100 µm. Red color indicates EdU-positive cells. d, e Cell apoptosis of A549 and H1975 cells with LARRPM stable overexpression and A549 and H1975or control cells was detected using caspase-3 activity assays (d) and TUNEL assays (e). Scale bar = 100 µm. Green color indicated TUNEL-positive cells. f, g Cell migration and invasion of A549 and H1975 cells with LARRPM stable overexpression or A549 and H1975 control cells was detected using transwell migration assay (f) and transwell invasion assay (g). Scale bar = 100 µm. Results are shown as mean ± SD based on three independent experiments. Asterisks indicate a significant difference at *P < 0.05, **P < 0.01, ***P < 0.001, by the Student’s t-test. CCK-8 Cell Counting Kit-8, Ctrl control, EdU 5-ethynyl-2′-deoxyuridine, oe overexpression, Tunel terminal deoxynucleotidyl transferase (TdT)-mediated dUTP nick end labeling