Fig. 5

Depletion of the LINC00240 reversed the roles of LARRPM in proliferation, apoptosis, migration and invasion of LUAD cells. a LARRPM and LINC00240 expression in A549 cells with LARRPM overexpression; LINC00240 depletion was detected by qRT-PCR. b, c Cell proliferation of A549 cells with LARRPM overexpression and LINC00240 depletion was detected using the CCK-8 assay (b) and EdU incorporation assay (c). Scale bar: 100 µm. Red color indicates the EdU-positive cells. d, e Cell apoptosis of A549 cells with LARRPM overexpression and LINC00240 depletion was detected using the caspase-3 activity assay (d) and TUNEL assay (e). Scale bar: 100 µm. Green color indicates TUNEL-positive cells. f, g Cell migration and invasion of A549 cells with LARRPM overexpression and LINC00240 depletion were detected using the transwell migration assay (f) and transwell invasion assay (g). Scale bar: 100 µm. Results are shown as the mean ± SD based on three independent experiments. Asterisks indicate a significant difference at *P < 0.05, **P < 0.01, ***P < 0.001; ns, not significant, by one-way ANOVA followed by Dunnett's multiple comparisons test