Fig. 8

Ectopic expression of LARRPM in LUAD cells attenuated the oncogenic roles of infiltrated M2 macrophages. a–f CM was collected from co-culture of macrophages with LARRPM overexpressed or control A549 cells, or collected from only A549 cells with LARRPM overexpression or A549 control cells. a, b Cell proliferation of A549 cells treated with the CM was detected using the CCK-8 assay (a) and EdU incorporation assay (b). Scale bar: 100 µm. Red color indicates EdU-positive cells. c, d Cell apoptosis of A549 cells treated with the CM was detected using the caspase-3 activity assay (c) and TUNEL assay (d). Scale bar: 100 µm. Green color indicates TUNEL-positive cells. e, f Cell migration and invasion of A549 cells treated with the CM was detected using the transwell migration assay (e) and transwell invasion assay (f). Scale bar: 100 µm. Results are shown as the mean ± SD based on three independent experiments. Asterisks indicate a significant difference at *P < 0.05, **P < 0.01, ns, not significant, by Student’s t-test. CM Conditioned medium