Fig. 4

MKNK2 was a novel target of miR-92a-2-5p in cardiomyocytes. A Schematic representation of the full-length 3’UTR of rat MKNK2 mRNA. #1—#7, potential binding sites of miR-92a-2-5p. B, C Dual-luciferase reporter assay verified the binding of miR-92a-2-5p and wild- or mutant‐type 3’-UTR of MKNK2. n = 3 in each group. **P < 0.01. D Representative images of western blot for MKNK2 protein in cardiomyocytes infected with sh-miR-92a-2-5p or Ad-miR-92a-2-5p. E, Integrated density analysis of immune bands using ImageJ software. β-actin was used as a loading control. n = 3 in each group. **P < 0.01. F Representative images of western blot for MKNK2 protein in cardiomyocytes after 24-h or 48-h high glucose stimulation. G Integrated density analysis of immune bands using ImageJ software. β-actin was used as a loading control. n = 3 in each group. **P < 0.01