Skip to main content
Fig. 5 | Cellular & Molecular Biology Letters

Fig. 5

From: Identification of COX4I2 as a hypoxia-associated gene acting through FGF1 to promote EMT and angiogenesis in CRC

Fig. 5

COX4I2 protein overexpression promotes CRC malignancy. a Analysis of IHC results and COX4I2 levels in CRC and control tissues (n = 30). b COX4I2 levels in tumor (T) and paired normal tissues (N) shown by western blotting (n = 30). c COX4I2 protein levels in normal human colonic epithelial cells and CRC cells lines. d Transfection efficiencies (%) shown by GFP expression and western blotting. (magnification: ×400, scale bars: 20 μm). e Clone formation in CRC cells transfected with the NC, sh-COX4I2, and oe-COX4I2 constructs (magnification: ×1, scale bars: 500 μm). f Mouse xenograft tumors (n = 6 mice per group). g Volumes of xenograft tumors measured twice a week. h Weights of xenograft tumors at completion of the study. i, j Correlation between IHC staining intensity for COX4I2 and Ki-67 in xenograft tumors (n = 24) and in CRC tissues (n = 30). Measures were taken from different samples. (magnification: ×100, scale bars: 100 μm). k FGF1 protein levels in culture supernatants of CRC cells 48 h after transfection, measured by ELISA. l FGF1 levels in mouse sera, measured by ELISA. Measurements were taken from distinct samples (n = 6). m IHC staining of FGF1 proteins in mouse xenograft tumor tissues (magnification: ×200, scale bars: 50 μm; magnification: ×400, scale bars: 20 μm). n COX4I2 and FGF1 protein levels in CRC and normal tissue (magnification: ×400, scale bars: 20 μm). o Correlation between COX4I2 and FGF1 in CRC. Statistical analyses were performed by the χ2 test (R Pearson correlation coefficient). Data are expressed as the mean ± standard error of the mean. Asterisks in all figure parts indicate significant difference at *P < 0.05, **P < 0.01, ***P < 0.001,. All IHC scores were repeated three times using a double-blind method. For the statistical analysis all experiments were repeated at least three times independently. GFP Green fluorescent protein, oe-COX4I2 overexpressing plasmid, RKO, SW480 cell lines, sh-COX4I2 shRNA-COX4I2 plasmid); for other Abbreviations, see Abbreviation list

Back to article page