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Fig. 3 | Cellular & Molecular Biology Letters

Fig. 3

From: 5-Methoxyflavone-induced AMPKα activation inhibits NF-κB and P38 MAPK signaling to attenuate influenza A virus-mediated inflammation and lung injury in vitro and in vivo

Fig. 3

Anti-IAV activity of 5-MF. A Immunofluorescence analysis was performed to detect the expression of viral NP in the lung tissues. B The fluorescence intensity for viral NP protein was quantified. C The cytotoxicity of 5-MF (0–200 μM) on A549 cells was measured using the MTT assay. D CPE on MDCK cells induced by three IV strains [A/PR/8/34 (H1N1), A/HK/8/68 (H3N2), and A/HK/Y280/97 (H9N2)] were visualized using an inverted light microscope after a 48-h incubation. E 5-MF reduced IAV-induced the formation of viral plaques in MDCK cells. F Western blot analysis was performed to detect the expression of RSAD2 in the H1N1 virus-infected A549 cells. G The relative protein band intensity of RSAD2 was quantitated using ImageJ software. H MDCK cells were pretreated with 5-MF for 4 h, and then infected with H1N1 virus for 1 h at 37 °C. MDCK cells were stained with acridine orange (4 μg/mL) for 10 min and analyzed by confocal microscope. ##p < 0.01, ###p < 0.001 relative to the control group; *p < 0.05, **p < 0.01, ***p < 0.001 relative to the virus group

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