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Fig. 4. | Cellular & Molecular Biology Letters

Fig. 4.

From: 5-Methoxyflavone-induced AMPKα activation inhibits NF-κB and P38 MAPK signaling to attenuate influenza A virus-mediated inflammation and lung injury in vitro and in vivo

Fig. 4.

5-MF reduced H1N1 virus-elevated the expression levels of proinflammatory mediators. A Immunofluorescence four-color staining showed the expression of IL-6 (yellow) and TNF-α (red) in epithelial cells (detected by EpCAM; green). Nuclei were stained with DAPI (blue). B The fluorescence intensities for IL-6 and TNF-α were quantified. C Immunofluorescence four-color staining showed the expression of CXCL8 (yellow) and CCL2 (red) in epithelial cells (detected by EpCAM; green). Nuclei were stained with DAPI (blue). D The fluorescence intensities for CXCL8 and CCL2 were quantified. E Protein expression of a series of proinflammatory mediators (IL-6, CXCL8, TNF-α, CXCL10, CCL2, CCL3, CCL4, and GM-CSF) in the culture supernatants was analyzed by Luminex assay. F Protein expression of COX-2 was analyzed by western blotting. G The relative protein band intensity of COX-2 was quantitated using ImageJ software. H The levels of PGE2 in the culture supernatants were measured by ELISA assay. #p < 0.05, ##p < 0.01, ###p < 0.001 relative to the control group; *p < 0.05, **p < 0.01, ***p < 0.001 relative to the virus group

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