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Fig. 1 | Cellular & Molecular Biology Letters

Fig. 1

From: Inhibition of CISD2 promotes ferroptosis through ferritinophagy-mediated ferritin turnover and regulation of p62–Keap1–NRF2 pathway

Fig. 1

CISD2 knockdown accelerates the ferroptotic cell death induced by Erastin. A Detection of lipid peroxides in CISD2 modified HT-1080 cells through BODIPY staining and confocal microscope. The fluorescence images are representative of three independent experiments; the green fluorescence intensity was positively correlated with the level of lipid peroxides. Scale bar: 50 μm. B Statistical column graph of relative intensity of green fluorescence from BODIPY staining. C Detection of the cellular free iron by RPA staining. The relative intensity of RPA was calculated and the statistical graph was obtained from three independent experiments. D Detection of the GSH content in control or CISD2-silenced cells with or without the treatment of Erastin. E Cell viability assay in control or CISD2-silenced HT1080 cells after the treatment with Erastin (7.5 µM) alone or together with the indicated chemicals (1 µM ferrostatin-1, 0.5 mM GSH, 100 µM DFO, 0.5 mM NAC, 0.5 µM Necro, 5 µM Z-VAD-FMK), the cell viability in control cells treated with vehicle was defined as 100%, relative cell viabilities were calculated, and statistical graph was obtained from three independent experiments. F Detection of lipid peroxidation by MDA assay kit; the relative MDA content was calculated. P < 0.05, P < 0.01 between indicated groups

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