Fig. 1

EVA1A protein and mRNA levels in HCC tissues and HCC cell lines. A IHC staining of TMEM166 expression in HCC tissues and paired distant non-cancerous tissues (NCT) distinguished with H&E staining. B RT-qPCR analysis of EVA1A mRNA levels in twenty-five pairs of HCC and NCT samples. C, D Western blotting analysis of the expression levels of the EVA1A protein in HCC and NCT samples and quantitative analysis of the blot bands of EVA1A with ImageJ v1.53 k. β-actin was used as loading control. E, F, G RT-qPCR analysis of EVA1A mRNA levels (E) and western blotting analysis of EVA1A protein levels (F, G) in the normal human hepatocyte L02 cells and HCC cell lines. PCDB-EVA1A, an unlabeled plasmid, was used as a positive control. Data are presented as means ± SD of triplicate experiments. ***P < 0.001