Fig. 6

Effect of overexpression EVA1A on mitotic progression and autophagy. Hccl-M3 cells transfected with EVA1A-GFP plasmid were synchronized at mitotic phase by 100 ng/ml nocodazole treatment for 14 h. 0 h (A), 0.5 h (B), 1 h (C) and 4 h (D) after removal of nocodazole, cells were fixed, stained with anti-β-Tubulin and DAPI, and imaged by confocal microscopy. Scale bars, 5 μm. (E) Hccl-M3 cells were transfected with GFP empty vector or EVA1A-GFP plasmid, 24 h later, cells were fixed, stained with anti-LC3 and DAPI, and imaged by confocal microscopy. Scale bars, 5 μm. (F) Hccl-M3 cells were transfected with Myc-tag vector or Myc-EVA1A plasmid, 24 h later, cells were lysed and subjected to western blot