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Fig. 7 | Cellular & Molecular Biology Letters

Fig. 7

From: Down-regulation of EVA1A by miR-103a-3p promotes hepatocellular carcinoma cells proliferation and migration

Fig. 7

Repression of miR-103a-3p induces HCC cells apoptosis through upregulating EVA1A/TP53 pathway. A Hccl-M3 cells were transfected with miR-103a-3p mimics, mimics control, miR-103a-3p inhibitor or inhibitor control. 72 h after transfection, the TP53 mRNA levels was detected by RT-qPCR. B The protein levels of TP53, BCL-2, BAX and EVA1A was detected by western blot. C Quantitative analysis of the blot bands of TP53, BCL-2, BAX and EVA1A. D RT-qPCR analysis of TP53 mRNA in Hccl-M3 cells overexpressing EVA1A. E Hccl-M3 cells were transfected with miR-103a-3p mimics alone or together with Myc-EVA1A plasmid, or transfected with miR-103a-3p inhibitors alone or together with siRNA of EVA1A.Then the expression of TP53 and EVA1A was detected by western blot. F Quantitative analysis of the blot bands of TP53 and EVA1A. G Hccl-M3 cells were transfected with miR-103a-3p inhibitor or siRNA of EVA1A, or both of them, then the apoptosis rate was assessed by Annexin V-FITC/PI staining and flow cytometry assay. H Statistical apoptosis rate of each group. Data are shown as means ± SD from three independent experiments. **P < 0.01, ***P < 0.001. I Western blot analysis of caspase-3 activation

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