Fig. 2

Establishment of Bm-MSCs senescence model by 30 μM H₂O₂ treatment. A The percentage of Sa-β-gal-positive cells were significantly decreased under H₂O₂ treatment. Scale bar, 20 μm. n = 5 biological replicates. B H₂O₂ treatment inhibited the cell proliferation of MSCs, n = 3. C The effect of H₂O₂ (30 μM) treatment on cell cycle phase by Flow cytometry analyses. D The expression of p21, p16 and p53 was down-regulated under H₂O₂ (n = 3). E The expression of Ki67 was down-regulated. At least 50 cells were statistically analyzed. F Clone expansion capability was reduced by H₂O₂ (n = 3)