Fig. 1
From: E2F1 promotes cell cycle progression by stabilizing spindle fiber in colorectal cancer cells
E2F1 is positively correlated with stathmin1 and TACC3 in pathological tissues derived from CRC patients. A Differential expression of genes in CRC cells with and without knockdown of E2F1 expression and screening for genes associated with mitotic spindle organization. The 51 screened genes were verified by qPCR. B qPCR analysis of relative mRNA levels encoded by these 51 genes in HCT116 cells with and without E2F1 knockdown for 48 h. C Differential expression of E2F1, stathmin1, and TACC3 in 104 pairs of CRC tumor and adjacent normal colorectal tissues, based on the TCGA database. Red color represents increased expression and green indicates reduced expression. Expression was normalized using (levelcase-meanrow)/SDrow. D Effects of cancer stage on levels of expression of E2F1, stathmin1, and TACC3 in 315 COAD and 166 READ cases from the TCGA database and analyzed by UALCAN (http://ualcan.path.uab.edu/analysis.html). The P-values (versus normal) were shown without indications and the P-values (versus Stage 1 and Stage 2) were indicated as S1 and S2 COAD colon adenocarcinoma, READ rectum adenocarcinoma E Correlations among E2F1, stathmin1, and TACC3 levels in 698 CRC (including COAD and READ) pathological tissue samples from the TCGA database, as determined by linear correlativity. Pearson’s test was used to analyze the correlations