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Fig. 2 | Cellular & Molecular Biology Letters

Fig. 2

From: E2F1 promotes cell cycle progression by stabilizing spindle fiber in colorectal cancer cells

Fig. 2

Upregulation of E2F1 promotes the expression of stathmin1 and TACC3 and facilitates cell cycle progression. A Total proteins were extracted from HT29, HCT8, DLD1, RKO, SW480, SW620, and HCT116 cells. The levels of E2F1, stathmin1, and TACC3 were determined by immunoblotting. B Correlations among E2F1, stathmin1, and TACC3 levels in the CRC cell lines shown in A. Pearson’s test was used to analyze the correlations. C Viability of the cells shown in A, as measured using MTS assays 0, 24, 48, and 72 h after cell seeding. D DLD1 cells were transfected with pCMV-E2F1 or pCMV-blank vectors (final vector concentrations, 2 μg/ml). HCT116 cells were transfected with E2F1-specific or control siRNA (final siRNA concentrations, 20 nM). Samples were harvested 48 h after transfection, and the levels of the indicated factors determined by immunoblotting. E Relative levels of expression of the proteins indicated in D. F DLD1 and HTC116 cells were transfected with vectors or siRNAs as described in D. Cell viability was measured using MTS assays 0, 24, 48, and 72 h after transfection

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