Fig. 6

Phosphorylation of stathmin1 at Ser16 facilitates the formation of TACC3/clathrin/α-tubulin complexes and enhances the stability of spindle fiber arrays. A Co-immunoprecipitation and immunoblot analyses of indicated factors in DLD1 cells transfected with pCMV-stathmin-FLAG or pCMV-stathmin1-M-S16-Ala-FLAG vectors (final vector concentrations, 2 μg/ml) for 48 h. The indicated factors were immunoprecipitated using Protein A/G beads conjugated with indicated antibodies. B Schematic representation of the stathmin1-TACC3-clathrin interaction. Phosphorylation of stathmin1 at Ser16 facilitated its interactions with TACC3 and clathrin. C Co-immunoprecipitation and immunoblot analyses of indicated factors in DLD1 cells transfected with vectors as shown in A for 48 h. The indicated factors were immunoprecipitated using Protein A/G beads conjugated with indicated antibodies. D Schematic representation of stathmin1-TACC3-clathrin-α-tubulin interactions. Phosphorylation of stathmin1 at Ser16 facilitated the formation of TACC3/clathrin/α-tubulin complexes and enhanced the stability of spindle fiber arrays